Homologous pairing between single-stranded DNA immobilized on a nitrocellulose membrane and duplex DNA is specific for RecA activity in bacterial crude extract.
Reaction between a circular single stranded and a linear double stranded DNA molecule (ssDNA and dsDNA) provides an efficient system to study recombination mediated by RecA protein. However, classical assays using reaction in solution require highly purified enzymes. This limits biochemical studies...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1993
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309861/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367282/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3653 |
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