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Sequence selective double strand DNA cleavage by peptide nucleic acid (PNA) targeting using nuclease S1.

A novel method for sequence specific double strand DNA cleavage using PNA (peptide nucleic acid) targeting is described. Nuclease S1 digestion of double stranded DNA gives rise to double strand cleavage at an occupied PNA strand displacement binding site, and under optimized conditions complete clea...

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Udgivet i:Nucleic Acids Res
Principais autores: Demidov, V, Frank-Kamenetskii, M D, Egholm, M, Buchardt, O, Nielsen, P E
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309471/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8502550/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.9.2103
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