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Expression, Purification, and Characterisation of Dehydroquinate Synthase from Pyrococcus furiosus
Dehydroquinate synthase (DHQS) catalyses the second step of the shikimate pathway to aromatic compounds. DHQS from the archaeal hyperthermophile Pyrococcus furiosus was insoluble when expressed in Escherichia coli but was partially solubilised when KCl was included in the cell lysis buffer. A purifi...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
SAGE-Hindawi Access to Research
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3092513/ https://ncbi.nlm.nih.gov/pubmed/21603259 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/134893 |
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