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Folding of DNA substrate-hairpin ribozyme domains: use of deoxy 4-thiouridine as an intrinsic photolabel.

Hairpin ribozymes derived from (-)sTRSV RNA exhibit substantial cleavage activity when wobble GU base pairs are introduced in place of the AU pairs normally involved in helices I and II between substrate and ribozyme. This finding prompted us to synthesize by in vitro transcription a new hairpin rib...

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Udgivet i:Nucleic Acids Res
Principais autores: Dos Santos, D V, Vianna, A L, Fourrey, J L, Favre, A
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 1993
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309093/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8441628/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.2.201
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