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Study of neurotrophin 3 signaling in primary cultured neurons using multiplex stable isotope labeling with amino acids in cell culture

Conventional stable isotope labeling with amino acids in cell culture (SILAC) requires extensive metabolic labeling of proteins, and therefore is difficult to apply to cells that do not divide or are unstable in SILAC culture. Using two different sets of heavy amino acids for labeling allows for str...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zhang, Guoan, Deinhardt, Katrin, Chao, Moses V., Neubert, Thomas A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3090507/
https://ncbi.nlm.nih.gov/pubmed/21370927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr200016n
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