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Evaluation of a PCR Assay for Quantitation of Rickettsia rickettsii and Closely Related Spotted Fever Group Rickettsiae

A spotted fever rickettsia quantitative PCR assay (SQ-PCR) was developed for the detection and enumeration of Rickettsia rickettsii and other closely related spotted fever group rickettsiae. The assay is based on fluorescence detection of SYBR Green dye intercalation in a 154-bp fragment of the rOmp...

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Détails bibliographiques
Auteurs principaux: Eremeeva, Marina E., Dasch, Gregory A., Silverman, David J.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2003
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC308968/
https://ncbi.nlm.nih.gov/pubmed/14662926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.12.5466-5472.2003
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