Evaluation of a PCR Assay for Quantitation of Rickettsia rickettsii and Closely Related Spotted Fever Group Rickettsiae

A spotted fever rickettsia quantitative PCR assay (SQ-PCR) was developed for the detection and enumeration of Rickettsia rickettsii and other closely related spotted fever group rickettsiae. The assay is based on fluorescence detection of SYBR Green dye intercalation in a 154-bp fragment of the rOmp...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Eremeeva, Marina E., Dasch, Gregory A., Silverman, David J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Chlamydiology and Rickettsiology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308968/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14662926/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.12.5466-5472.2003
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