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Cys-X Scanning for Expansion of Active-site Residues and Modulation of Catalytic Functions in a Glutathione Transferase

We propose Cys-X scanning as a semisynthetic approach to engineer the functional properties of recombinant proteins. As in the case of Ala scanning, key residues in the primary structure are identified, and one of them is replaced by Cys via site-directed mutagenesis. The thiol of the residue introd...

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Autors principals: Norrgård, Malena A., Hellman, Ulf, Mannervik, Bengt
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3089530/
https://ncbi.nlm.nih.gov/pubmed/21454564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.230078
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