Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease

Unlike orthodox Type II restriction endonucleases that are homodimers and interact with the palindromic 4–8-bp DNA sequences, BcnI is a monomer which has a single active site but cuts both DNA strands within the 5′-CC↓CGG-3′/3′-GGG↓CC-5′ target site (‘↓’ designates the cleavage position). Therefore,...

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Bibliografiske detaljer
Main Authors: Kostiuk, Georgij, Sasnauskas, Giedrius, Tamulaitiene, Giedre, Siksnys, Virginijus
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2011
Fag:
Nucleic Acid Enzymes
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3089477/
https://ncbi.nlm.nih.gov/pubmed/21227928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1351
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