Fluorescent labeling of tetracysteine-tagged proteins in intact cells

In this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder–ethanedithiol (FlAsH-EDT(2)). Generation of the tetracysteine-tagged protein construct by itself is not described, as this is a protein-speci...

Celý popis

Uloženo v:
Podrobná bibliografie
Hlavní autoři: Hoffmann, Carsten, Gaietta, Guido, Zürn, Alexander, Adams, Stephen R, Terrillon, Sonia, Ellisman, Mark H, Tsien, Roger Y, Lohse, Martin J
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3086663/
https://ncbi.nlm.nih.gov/pubmed/20885379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.129
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky