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Fluorescent labeling of tetracysteine-tagged proteins in intact cells
In this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder–ethanedithiol (FlAsH-EDT(2)). Generation of the tetracysteine-tagged protein construct by itself is not described, as this is a protein-speci...
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| Autors principals: | , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3086663/ https://ncbi.nlm.nih.gov/pubmed/20885379 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.129 |
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