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Trypsin is the Primary Mechanism by which the (18)O Isotopic Label is Lost in Quantitative Proteomic Studies

Labeling with (18)O is currently one of the most commonly used methods for incorporating a stable isotopic label into samples for comparative proteomic studies. In this approach, isotopic labeling involves the enzymatic digestion, typically performed with trypsin, of a protein population in (18)O wa...

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Détails bibliographiques
Auteurs principaux: Angel, Peggi M., Orlando, Ron
Format: Artigo
Langue:Inglês
Publié: 2006
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3086039/
https://ncbi.nlm.nih.gov/pubmed/17046705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.08.036
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