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A RADIATION TARGET METHOD FOR SIZE DETERMINATION OF SUPERCOILED PLASMID DNA

Supercoiled DNA plasmids were exposed in the frozen state to high energy electrons. Surviving supercoiled molecules were separated from their degradation products (e.g., open circle and linear forms) by agarose gel electrophoresis and subsequently quantified by staining and image analysis. Complex s...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Anchordoquy, T. J., Molina, M. dC., Kempner, E. S.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2008
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC3085921/
https://ncbi.nlm.nih.gov/pubmed/19028447
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.10.049
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