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A RADIATION TARGET METHOD FOR SIZE DETERMINATION OF SUPERCOILED PLASMID DNA
Supercoiled DNA plasmids were exposed in the frozen state to high energy electrons. Surviving supercoiled molecules were separated from their degradation products (e.g., open circle and linear forms) by agarose gel electrophoresis and subsequently quantified by staining and image analysis. Complex s...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2008
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3085921/ https://ncbi.nlm.nih.gov/pubmed/19028447 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.10.049 |
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