Shot-Noise Limited Single-Molecule FRET Histograms: Comparison between Theory and Experiments

We describe a simple approach and present a straightforward numerical algorithm to compute the best fit shot-noise limited proximity ratio histogram (PRH) in single-molecule fluorescence resonant energy transfer diffusion experiments. The key ingredient is the use of the experimental burst size dist...

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Autori principali: Nir, Eyal, Michalet, Xavier, Hamadani, Kambiz M., Laurence, Ted A., Neuhauser, Daniel, Kovchegov, Yevgeniy, Weiss, Shimon
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2006
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3085016/
https://ncbi.nlm.nih.gov/pubmed/17078646
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp063483n
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