Chemically defined conditions for human iPS cell derivation and culture

We reexamine the individual components for human ES and iPS cell culture, and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces, and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in eith...

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Hauptverfasser: Chen, Guokai, Gulbranson, Daniel R., Hou, Zhonggang, Bolin, Jennifer M., Ruotti, Victor, Probasco, Mitchell D., Smuga-Otto, Kimberly, Howden, Sara E., Diol, Nicole R., Propson, Nicholas E., Wagner, Ryan, Lee, Garrett O., Antosiewicz-Bourget, Jessica, Teng, Joyce M. C., Thomson, James A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3084903/
https://ncbi.nlm.nih.gov/pubmed/21478862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1593
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