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High Affinity scFv–Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells

We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustra...

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Main Authors: Iyer, Gopal, Michalet, Xavier, Chang, Yun-Pei, Pinaud, Fabien F., Matyas, Stephanie E., Payne, Gregory, Weiss, Shimon
格式: Artigo
語言:Inglês
出版: 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3084663/
https://ncbi.nlm.nih.gov/pubmed/19053789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl8032284
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