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High Affinity scFv–Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustra...
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Main Authors: | , , , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
2008
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3084663/ https://ncbi.nlm.nih.gov/pubmed/19053789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl8032284 |
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