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Tracking Single Proteins in Live Cells Using Single-Chain Antibody Fragment-Fluorescent Quantum Dot Affinity Pair

Quantum dots (QDs) are extremely bright fluorescent imaging probes that are particularly useful for tracking individual molecules in living cells. Here, we show how a two-component system composed of a high-affinity single-chain fragment antibody and its cognate hapten (fluorescein) can be utilized...

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Hlavní autoři: Iyer, Gopal, Michalet, Xavier, Chang, Yun-Pei, Weiss, Shimon
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3405731/
https://ncbi.nlm.nih.gov/pubmed/20627153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)75003-5
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