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Tracking Single Proteins in Live Cells Using Single-Chain Antibody Fragment-Fluorescent Quantum Dot Affinity Pair

Quantum dots (QDs) are extremely bright fluorescent imaging probes that are particularly useful for tracking individual molecules in living cells. Here, we show how a two-component system composed of a high-affinity single-chain fragment antibody and its cognate hapten (fluorescein) can be utilized...

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Detalhes bibliográficos
Main Authors: Iyer, Gopal, Michalet, Xavier, Chang, Yun-Pei, Weiss, Shimon
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3405731/
https://ncbi.nlm.nih.gov/pubmed/20627153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(10)75003-5
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