Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes.
We describe codon cassette mutagenesis, a simple method of mutagenesis that uses universal mutagenic cassettes to deposit single codons at specific sites in double-stranded DNA. A target molecule is first constructed that contains a blunt, double-strand break at the site targeted for mutagenesis. A...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1994
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308034/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202358/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.9.1593 |
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