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Purification, crystallization and preliminary X-ray diffraction analysis of the seryl-tRNA synthetase from Candida albicans

The seryl-tRNA synthetase (SerRS) from Candida albicans exists naturally as two isoforms resulting from ambiguity in the natural genetic code. Both enzymes were crystallized by the sitting-drop vapour-diffusion method using 3.2–3.4 M ammonium sulfate as precipitant. The crystals belonged to the hexa...

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Main Authors: Rocha, Rita, Barbosa Pereira, Pedro José, Santos, Manuel A. S., Macedo-Ribeiro, Sandra
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2010
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079998/
https://ncbi.nlm.nih.gov/pubmed/21206050
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110048542
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