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Expression, purification, crystallization and preliminary X-ray analysis of the KaiC-like protein PH0187 from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

KaiC is the central protein in the circadian rhythm in cyanobacteria. The 28 kDa KaiC-like protein PH0187 from the hyperthermophilic archaeon Pyrococcus horikoshii was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293 K. Crystals of PH0187...

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Bibliografski detalji
Glavni autori: Kang, Hee-Jin, Kubota, Keiko, Miyazono, Ken-ichi, Tanokura, Masaru
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079995/
https://ncbi.nlm.nih.gov/pubmed/21206047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110048426
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