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Expression, high-pressure refolding, purification, crystallization and preliminary X-ray analysis of a novel single-strand-specific 3′–5′ exonuclease PhoExo I from Pyrococcus horikoshii OT3

PhoExo I is a single-strand-specific 3′–5′ exonuclease from Pyrococcus horikoshii OT3 and is thought to be involved in a Thermococcales-specific DNA-repair pathway. The recombinant PhoExo I protein was produced as inclusion bodies in Escherichia coli cells. Solubilization of the inclusion bodies was...

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Main Authors: Miyazono, Ken-ichi, Tsutsumi, Kanae, Ishino, Yoshizumi, Tanokura, Masaru
格式: Artigo
語言:Inglês
出版: International Union of Crystallography 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4118808/
https://ncbi.nlm.nih.gov/pubmed/25084386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14012734
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