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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of NifH2 from Methanocaldococcus jannaschii

Nitrogenases are protein complexes that are only found in Azotobacter and are required for biological nitrogen fixation. They are made up of a nitrogenase, which is a NifD(2)/NifK(2) heterotetramer, and a nitrogenase reductase, which is a homodimer of NifH. Many homologues of nitrogenase have been f...

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Detalhes bibliográficos
Main Authors: Huang, Kai, Ma, Jinming, Yuan, Ye, Gao, Yongxiang
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079992/
https://ncbi.nlm.nih.gov/pubmed/21206044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110048104
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