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Cloning, expression, purification, crystallization and preliminary crystallographic analysis of NifH2 from Methanocaldococcus jannaschii

Nitrogenases are protein complexes that are only found in Azotobacter and are required for biological nitrogen fixation. They are made up of a nitrogenase, which is a NifD(2)/NifK(2) heterotetramer, and a nitrogenase reductase, which is a homodimer of NifH. Many homologues of nitrogenase have been f...

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Библиографические подробности
Главные авторы: Huang, Kai, Ma, Jinming, Yuan, Ye, Gao, Yongxiang
Формат: Artigo
Язык:Inglês
Опубликовано: International Union of Crystallography 2010
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3079992/
https://ncbi.nlm.nih.gov/pubmed/21206044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110048104
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