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Genome-wide identification of Ago2 binding sites from mouse embryonic stem cells with and without mature microRNAs
MicroRNAs (miRNAs) are 19-22nt non-coding RNAs that post-transcriptionally regulate mRNA targets. To identify endogenous miRNA binding sites, we performed photo-crosslinking immunoprecipitation using Ago2 antibodies, followed by deep-sequencing of RNAs (CLIP-seq) in mouse embryonic stem cells (mESCs...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3078052/ https://ncbi.nlm.nih.gov/pubmed/21258322 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.1991 |
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