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Subunit Dimers of α-Hemolysin Expand the Engineering Toolbox for Protein Nanopores

Staphylococcal α-hemolysin (αHL) forms a heptameric pore that features a 14-stranded transmembrane β-barrel. We attempted to force the αHL pore to adopt novel stoichiometries by oligomerizing subunit dimers generated by in vitro transcription and translation of a tandem gene. However, in vitro trans...

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Autors principals: Hammerstein, Anne F., Jayasinghe, Lakmal, Bayley, Hagan
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077633/
https://ncbi.nlm.nih.gov/pubmed/21324910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M111.218164
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