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Use of a selection technique to identify the diversity of binding sites for the yeast RAP1 transcription factor.

We have used the technique known as selected and amplified binding (SAAB) to isolate binding sites for the yeast transcription factor RAP1 from a degenerate pool of oligonucleotides. A total of 47 sequences were isolated, of which two were shown to be contaminating non-RAP1 binding sites. After excl...

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Detalhes bibliográficos
Main Authors: Graham, I R, Chambers, A
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC307761/
https://ncbi.nlm.nih.gov/pubmed/8121795
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