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A direct selection strategy for shotgun cloning and sequencing in the bacteriophage M13.
A new cloning strategy is described which utilizes direct selection of recombinants for shotgun sequencing in the filamentous bacteriophage M13. Direct selection is accomplished by insertional inactivation of the M13 gene X protein, a powerful inhibitor of phage-specific DNA synthesis when overprodu...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1994
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307752/ https://ncbi.nlm.nih.gov/pubmed/8127645 |
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