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A direct selection strategy for shotgun cloning and sequencing in the bacteriophage M13.

A new cloning strategy is described which utilizes direct selection of recombinants for shotgun sequencing in the filamentous bacteriophage M13. Direct selection is accomplished by insertional inactivation of the M13 gene X protein, a powerful inhibitor of phage-specific DNA synthesis when overprodu...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Guilfoyle, R A, Smith, L M
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1994
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC307752/
https://ncbi.nlm.nih.gov/pubmed/8127645
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