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A direct selection strategy for shotgun cloning and sequencing in the bacteriophage M13.
A new cloning strategy is described which utilizes direct selection of recombinants for shotgun sequencing in the filamentous bacteriophage M13. Direct selection is accomplished by insertional inactivation of the M13 gene X protein, a powerful inhibitor of phage-specific DNA synthesis when overprodu...
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| Vydáno v: | Nucleic Acids Res |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Oxford University Press
1994
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| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307752/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8127645/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.1.100 |
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