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Microwave assisted acid cleavage for denaturation and proteolysis of intact human adenovirus

Microwave assisted acid cleavage was applied directly to intact adenovirus type 5 to achieve denaturation and proteolysis in a single reaction. The speed of the digestion, coupled with the simplicity of MALDI analysis, allowed peptide products to be profiled in less than 5 min. Identification of pep...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Fenselau, Catherine, Laine, Olli, Swatkoski, Stephen
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076687/
https://ncbi.nlm.nih.gov/pubmed/21499518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ijms.2010.05.026
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