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Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is...

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Detalhes bibliográficos
Main Authors: Zakharchenko, Olena, Greenwood, Christina, Alldridge, Louise, Souchelnytskyi, Serhiy
Formato: Artigo
Idioma:Inglês
Publicado em: Libertas Academica 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076014/
https://ncbi.nlm.nih.gov/pubmed/21494400
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4137/BCBCR.S6263
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