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Proteomic Studies of Syk-Interacting Proteins Using a Novel Amine-Specific Isotope Tag and GFP Nanotrap

Green fluorescent protein (GFP) and variants have become powerful tools to study protein localization, interactions, and dynamics. We present here a mass spectrometry-based proteomics strategy to examine protein–protein interactions using anti-GFP single-chain antibody V(H)H in a combination with a...

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Detalhes bibliográficos
Main Authors: Galan, Jacob A., Paris, Leela L., Zhang, Hua-jie, Adler, Jacob, Geahlen, Robert L., Tao, W. Andy
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074380/
https://ncbi.nlm.nih.gov/pubmed/21472591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-010-0030-7
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