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Quantitative Proteomics Employing Primary Amine Affinity Tags

A proteomics-based method using stable isotope labeling to assess the relative abundance of peptides or proteins is described. Bradykinin and carbonic anhydrase were labeled with sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopropionate, a membrane impermeant reagent that is reactive with primary...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hoang, Van M., Conrads, Thomas P., Veenstra, Timothy D., Blonder, Josip, Terunuma, Atsushi, Vogel, Jonathan C., Fisher, Robert J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The Association of Biomolecular Resource Facilities 2003
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2279946/
https://ncbi.nlm.nih.gov/pubmed/13678152
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