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Tracking F plasmid TraI relaxase processing reactions provides insight into F plasmid transfer

Early in F plasmid conjugative transfer, the F relaxase, TraI, cleaves one plasmid strand at a site within the origin of transfer called nic. The reaction covalently links TraI Tyr16 to the 5′-ssDNA phosphate. Ultimately, TraI reverses the cleavage reaction to circularize the plasmid strand. The joi...

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Bibliographic Details
Main Authors: Dostál, Lubomír, Shao, Sichen, Schildbach, Joel F.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2011
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3074121/
https://ncbi.nlm.nih.gov/pubmed/21109533
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq1137
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