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An efficient method for the production of isotopically enriched cholesterol for NMR
(13)C-Cholesterol was produced with high efficiency by a genetically engineered yeast strain. The method produces ∼1 mg of cholesterol per gram of glucose using 100 ml of culture medium. Uniform 94% enrichment where the most abundant product is the fully enriched isotopomer (u-(13)C(27)) is obtained...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Biochemistry and Molecular Biology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3073474/ https://ncbi.nlm.nih.gov/pubmed/21357620 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D014209 |
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