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Quantification of DNA-protein interaction by UV crosslinking.

Measurement of the affinity of a protein for a promoter sequence is critical when assessing its potential to regulate transcription. Here we report that the DNA protein crosslinking (DPC) assay can be used to measure affinity, amount and molecular weight of DNA binding proteins to specific and non-s...

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Main Authors: Molnar, G, O'Leary, N, Pardee, A B, Bradley, D W
格式: Artigo
語言:Inglês
出版: 1995
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC307194/
https://ncbi.nlm.nih.gov/pubmed/7667109
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