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Mapping the path of the nascent peptide chain through the 23S RNA in the 50S ribosomal subunit.
Peptides of different lengths encoded by suitable mRNA fragments were biosynthesized in situ on Escherichia coli ribosomes. The peptides carried a diazirine derivative bound to their N-terminal methionine residue, which was photoactivated whilst the peptides were still attached to the ribosome. Subs...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1995
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307040/ https://ncbi.nlm.nih.gov/pubmed/7630714 |
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