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A sensitive mass-spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples

The recent discovery of 5-hydroxymethyl-cytosine (5hmC) in embryonic stem cells and post-mitotic neurons has triggered the need for quantitative measurements of both 5-methyl-cytosine (5mC) and 5hmC in the same sample. We have developed a method using liquid chromatography electrospray ionization ta...

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Detalhes bibliográficos
Main Authors: Le, Thuc, Kim, Kee-Pyo, Fan, Guoping, Faull, Kym F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3070205/
https://ncbi.nlm.nih.gov/pubmed/21272560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2011.01.026
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