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Tuning the affinity of aminoacyl-tRNA to elongation factor Tu for optimal decoding
To better understand why aminoacyl-tRNAs (aa-tRNAs) have evolved to bind bacterial elongation factor Tu (EF-Tu) with uniform affinities, mutant tRNAs with differing affinities for EF-Tu were assayed for decoding on Escherichia coli ribosomes. At saturating EF-Tu concentrations, weaker-binding aa-tRN...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2011
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3069205/ https://ncbi.nlm.nih.gov/pubmed/21402928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1102128108 |
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