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Understanding the Sequence Specificity of tRNA Binding to EF-Tu using tRNA Mutagenesis
Measuring the binding affinities of 42 single base pair mutants in the acceptor and T-stems of S. cerevisiae tRNA(Phe) to T. thermophilus EF-Tu revealed that most of the specificity for tRNA occurs at the 49-65, 50-64, and 51-63 base pairs. Introducing the same mutations at the three positions into...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2779119/ https://ncbi.nlm.nih.gov/pubmed/19452597 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.01.021 |
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