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Efficient Generation of Unmarked Deletions in Legionella pneumophila
Unmarked gene deletions facilitate studies of Legionella pneumophila multicomponent processes, such as motility and exonuclease activity. For this purpose, FRT-flanked alleles constructed in Escherichia coli using λ-Red recombinase were transferred to L. pneumophila by natural transformation. Resist...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2011
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3067435/ https://ncbi.nlm.nih.gov/pubmed/21296953 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02904-10 |
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