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Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene.

A new approach to determining the sequence of cloned DNA is described. Unique regions near each end of the transposable element gamma-delta provide a pair of "portable" primer-specific sites for bidirectional sequencing by the dideoxy chain termination method. A set of gamma-delta insertio...

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Bibliografske podrobnosti
Main Authors: Liu, L, Whalen, W, Das, A, Berg, C M
Format: Artigo
Jezik:Inglês
Izdano: 1987
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC306480/
https://ncbi.nlm.nih.gov/pubmed/2825136
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