Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene.
A new approach to determining the sequence of cloned DNA is described. Unique regions near each end of the transposable element gamma-delta provide a pair of "portable" primer-specific sites for bidirectional sequencing by the dideoxy chain termination method. A set of gamma-delta insertions positio...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1987
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| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306480/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2825136/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.22.9461 |
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