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When Pigs Fly: Immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis

In vivo mutation assays based on the Pig-a null phenotype, that is, the absence of cell surface glycosylphosphatidylinositol (GPI) anchored proteins such as CD59, have been described. This work has been accomplished with hematopoietic cells, most often rat peripheral blood erythrocytes (RBCs) and re...

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Autori principali: Dertinger, Stephen D., Bryce, Steven M., Phonethepswath, Souk, Avlasevich, Svetlana L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3064711/
https://ncbi.nlm.nih.gov/pubmed/21277384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mrgentox.2011.01.009
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