Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.

A method is described to synthesize small RNAs of defined length and sequence using T7 RNA polymerase and templates of synthetic DNA which contain the T7 promoter. Partially single stranded templates which are base paired only in the -17 to +1 promoter region are just as active in transcription as l...

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Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Milligan, J F, Groebe, D R, Witherell, G W, Uhlenbeck, O C
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1987
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC306405/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3684574/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.21.8783
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