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Nucleotide sequence of the DdeI restriction-modification system and characterization of the methylase protein.
The DdeI restriction-modification system was previously cloned and has been maintained in E. coli on two separate and compatible plasmids (1). The nucleotide sequence of the endonuclease and methylase genes has now been determined; it predicts proteins of 240 amino acids, Mr = 27,808, and 415 amino...
Sparad:
| Huvudupphovsmän: | , , , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1987
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306357/ https://ncbi.nlm.nih.gov/pubmed/2823226 |
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