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Comparing protein–ligand interactions in solution and single crystals by Raman spectroscopy
By using a Raman microscope, we show that it is possible to probe the conformational states in protein crystals and crystal fragments under growth conditions (in hanging drops). The flavin cofactor in the enzyme para-hydroxybenzoate hydroxylase can assume two conformations: buried in the protein mat...
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| Главные авторы: | , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
The National Academy of Sciences
2001
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC30597/ https://ncbi.nlm.nih.gov/pubmed/11248022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.061029598 |
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