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Analysis of trp repressor-operator interaction by filter binding.

A filter binding assay was developed that allows measurement of specific binding of trp repressor to operator DNA. The most important feature of this procedure is the concentration and type of salt present in the binding buffer. Using this assay the dissociation constant of the repressor-operator co...

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Detalhes bibliográficos
Main Authors: Klig, L S, Crawford, I P, Yanofsky, C
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC305965/
https://ncbi.nlm.nih.gov/pubmed/3299270
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