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High level production and rapid purification of the E. coli trp repressor.
Two small, multicopy, expression plasmids were constructed that permit convenient insertion of trpR, the structural gene for the trp repressor of Escherichia coli, with its natural ribosome binding site or adjacent to the ribosome binding site for the trp leader peptide. In these plasmids trpR is po...
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| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1986
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC311819/ https://ncbi.nlm.nih.gov/pubmed/3534793 |
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