Efficient methods for attachment of thiol specific probes to the 3'-ends of synthetic oligodeoxyribonucleotides.
Methodology is described for the synthesis of DNA oligomers containing a free 3'-thiol group which can be selectively crosslinked with a wide variety of probes. This chemistry is compatible with both phosphotriester and phosphoramidite solid phase chemistry. Moreover, the sulphydryl group is introdu...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1987
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3601673/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.13.5305 |
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