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Efficient methods for attachment of thiol specific probes to the 3'-ends of synthetic oligodeoxyribonucleotides.
Methodology is described for the synthesis of DNA oligomers containing a free 3'-thiol group which can be selectively crosslinked with a wide variety of probes. This chemistry is compatible with both phosphotriester and phosphoramidite solid phase chemistry. Moreover, the sulphydryl group is in...
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| Publié dans: | Nucleic Acids Res |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Oxford University Press
1987
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| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305963/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3601673/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/15.13.5305 |
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