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A novel assembly mechanism for the DNA polymerase III holoenzyme DnaX complex: association of δδ′ with DnaX(4) forms DnaX(3)δδ′

We have constructed a plasmid-borne artificial operon that expresses the six subunits of the DnaX complex of Escherichia coli DNA polymerase III holoenzyme: τ, γ, δ, δ′, χ and ψ. Induction of this operon followed by assembly in vivo produced two τγ mixed DnaX complexes with stoichiometries of τ(1)γ(...

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Publicat a:EMBO J
Autors principals: Pritchard, Arthur E., Dallmann, H.Garry, Glover, Bradley P., McHenry, Charles S.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2000
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305859/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11101526/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.23.6536
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